Types of chromatographic methods - LinkedIn SlideShare.
Analysis of Chromatography Methods. Analysis of Chromatography Methods Chromatography is the method of separating chemical substances that makes uses of the relative rates at which they are adsorbed from a moving stream of gas or liquid on a stationary substance, which is usually a finely divided solid, a sheet of filter material, or a thin film of a liquid on the surface of a solid.
There are different ways to separate mixtures, for example by filtration, crystallisation, distillation or chromatography. The method chosen depends upon the type of mixture.
Introduction. High performance liquid chromatography 214 is the most widely used of all of the analytical separation techniques. The reasons for the popularity of the method is its sensitivity, ready adaptability to accurate quantitative determinations, suitability for separating non-volatile species or thermally fragile ones, wide spread applicability to substance that are of prime interest.
Chromatography - Chromatography - Methods: Chromatographic methods are classified according to the following criteria: (1) geometry of the system, (2) mode of operation, (3) retention mechanism, and (4) phases involved. The mobile and stationary phases of chromatographic systems are arranged in such a way that migration is along a coordinate much longer than its width.
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For a liquid chromatographic method, the following sequence has proven to be useful while validating: Selectivity of standards (optimizing separation and detection of standard mixtures if selectivity is insufficient) Linearity, limit of quantitation, limit of detection, range Repeatability (short-term precision) of retention times and peak areas Intermediate precision Selectivity with real.
In all chromatographic methods, a sample is first applied onto a stationary material that absorbs or adsorbs the sample: adsorption is when molecules or ions in a sample adhere to a surface, while absorption is when the sample particles penetrate into the material. This material is called the stationary phase. After adsorption, the sample is then transported by a liquid or gas in one direction.